Regulatory

Part:BBa_K654096:Design

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-09-26)


E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mistake in primer design caused "fusion" part appearance, kept as it is a unique new promoter


Source

Primer Annealing

References